Total Testosterone Testing

This page explains the total testosterone testing, what it measures, and how to arrange it. Total testosterone testing measures the amount of testosterone in your blood, including both the portion attached to proteins and the small amount that circulates freely. It is commonly ordered to evaluate symptoms such as low sex drive, fatigue, infertility, or delayed or early puberty, and to monitor hormone therapy.

Sample type
blood
Category
Hormone
Turnaround
1-3 business days
Price
$49
Referral
Not required

Total testosterone testing measures the amount of testosterone in your blood, including both the portion attached to proteins and the small amount that circulates freely. It is commonly ordered to evaluate symptoms such as low sex drive, fatigue, infertility, or delayed or early puberty, and to monitor hormone therapy.

What it measures

  • Total testosterone — The combined amount of testosterone in your blood, bound and unbound.
  • Reference range — A lab-provided range showing where most healthy results fall, based on age and sex.
  • Collection time — Testosterone levels are usually highest in the morning, so timing is often noted.

Who it is for

A doctor or other clinician typically orders this test when symptoms suggest a hormone imbalance or when monitoring treatment. It may also be ordered as part of a broader evaluation of puberty, fertility, or sexual health.

Preparation

Morning collection is often preferred because testosterone levels tend to be higher then. Fasting is not usually required, but follow any specific instructions from your clinician or lab.

Results and what they mean

Results are reported as a number with a reference range, and your clinician interprets them alongside your symptoms, age, and other tests. A single result outside the range does not necessarily mean there is a problem. This is general information only and not medical advice; discuss your results with a qualified clinician.

How to order this test

  1. Choose the test and place a confidential order online.
  2. Select a certified collection site and pick a time that suits you.
  3. Have your specimen collected — most draws take only a few minutes.
  4. Review results in a secure portal, typically within 1–3 days.

Preparation, hours, and pricing vary by site; confirm details directly with the lab before your visit.

Availability by state

Direct-access online ordering for the total testosterone testing is available in most states. It is currently unavailable in 6 states:

Everywhere else you can order confidentially and collect your sample at a certified site. Browse by state →

Frequently asked questions

Do I need to fast before this test?

Fasting is usually not required. Your clinician or lab will tell you if any preparation is needed.

Why is the test often done in the morning?

Testosterone levels tend to be higher in the morning, so a morning sample can give a more consistent result.

Can one test result tell me if I have a hormone problem?

No. A single result is only one piece of information. Your clinician considers symptoms, repeat tests, and other findings before drawing conclusions.

Is this test the same as a free testosterone test?

No. Total testosterone measures all testosterone in the blood, while free testosterone measures only the unbound portion.

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